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Extraction

Edited by lukas.scharer on 19. October 2012 - 9:39

Extraction equipment

  • two beakers (preferably with screwcap lids, which allows better mixing)
  • a table spoon
  • a funnel
  • filtered seawater (preferably from the sample location)
  • MgCl2 solution (as an anaesthetic, at 71g/l for normal strength sea water)
  • always close your MgCl2 container properly. It is highly hygroscopic.
  • large and small petri dishes
  • nets (63 or 100µm mesh size)

Collection

Edited by lukas.scharer on 19. October 2012 - 5:58

Sampling Equipment

  • bucket
  • cups with a screw cap, such as urine cups
  • spoon
  • long knife
  • refractometer (to measure the salinity)
  • waterproof marker
  • sampling site description sheet
  • pencil
  • camera
  • GPS
  • camera
  • scoop (a device that allows to collect species that live on aquatic vegetation)

General Advice

Information for New Users

Edited by lukas.scharer on 25. January 2010 - 16:56

Dear New User,

You have recently registered for a user name on http://macrostomorpha.info and this page explains some of the basic features of the site.

First, you need to know that this page uses a content management environment, which was set up by the EDIT (European Institute of Distributed Taxonomy) Initiative. The system has six different levels of users:

Adding biblio content

Edited by lukas.scharer on 20. January 2010 - 8:10

How do I create a bibliographic citation?

Hover with the mouse over 'biblio' in the 'Content' menu on the left sidebar and click on the 'Add a biblio node' icon which appears to the right (the plus sign).

Select the 'Publication Type' and enter the required data (if you use EndNote you can use the paste function uising BibTex formated data).

Click on "Preview" to check the biblio entry and on "Submit" to save it.

About the Image Galleries

Edited by lukas.scharer on 24. July 2009 - 13:20

There are currently a number of image galleries, each of which serves a different purpose:

About This Site

Edited by lukas.scharer on 24. July 2009 - 12:43

This site is intended as an online repository for taxonomic and phylogenetic information on the Macrostomorpha, a basal group of free-living flatworms, with particular emphasis for the genus Macrostomum. According to the current phylogenies the Macrostomorpha are the most basal taxon of the Rhabditophora, which encompass all free-living flatworms (except the Acoelomorpha and the Catenulida) and all the parasitic flatworms.

About the Specimens

Edited by lukas.scharer on 28. April 2009 - 8:49

Specimens represent type material, that has either been documented digitally, from which DNA samples exist and/or have been analysed, from which there are whole mount preparations, and/or from which there is material available in embedded of serially sectioned form. If some of this material has been deposited elsewhere, the specimen record includes these other catalog numbers.

The code of a specimen record consists of three parts:
- the Institution Code (e.g. MTP for Macrostomum Taxonomy and Phylogeny)
- the Collection Code (e.g. LS for Lukas Schärer, the person holding the collection)

Fixation and Preservation

Edited by lukas.scharer on 21. December 2008 - 16:51

There are many ways of fixing and preserving free-living flatworms. The protocols I give here are only suggestions, which are largely based on suggestions of Willi Salvenmoser (Univ. Innsbruck) for histological preservation, and suggestions of Tom Artois (Univ. Hasselt) for whole mount preservation.

Fixation for histology:

MTP LS 518 Images and Movies

Submitted by lukas.scharer on 10. December 2008 - 14:10


Sending Samples

Edited by lukas.scharer on 5. December 2008 - 8:46

On this page I briefly explain how to best send living worms and DNA samples through the regular mail.

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